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  • Access full-text academic articles: J-STAGE is an online platform for Japanese academic journals.
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  • pH-dependent interaction of rhodopsin with cyanidin-3-glucoside. 2. Functional aspects.

    PubMed

    Tirupula, Kalyan C; Balem, Fernanda; Yanamala, Naveena; Klein-Seetharaman, Judith

    2009-01-01

    Anthocyanins are a class of phytochemicals that confer color to flowers, fruits, vegetables and leaves. They are part of our regular diet and serve as dietary supplements because of numerous health benefits, including improved vision. Recent studies have shown that the anthocyanin cyanidin-3-O-glucoside (C3G) increased regeneration of the dim-light photoreceptorrhodopsin (Matsumoto et al. [2003] J. Agric. Food Chem., 51, 3560-3563). In an accompanying study (Yanamala et al. [2009] Photochem. Photobiol.), we show that C3G directly binds to rhodopsin in a pH-dependent manner. In this study, we investigated the functional consequences of C3G binding to rhodopsin. As observed previously in rod outer segments, regeneration of purified rhodopsin in detergent micelles is also accelerated in the presence of C3G. Thermal denaturation and stability studies using circular dichroism, fluorescence and UV/visible absorbance spectroscopy show that C3G exerts a destabilizing effect on rhodopsin structure while it only modestly alters G-protein activation and the rates at which the light-activated Metarh


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  • Refill Magazine 4

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    23/9/04

    5:27 AM

    Page 1


    23/9/04

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    Refill ZeroFour Contents

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    INKHEADS RAY PARLA WONDERWALL ANDY HOWELL MAINFRAME SSUR INVISIBLE MAN IOSIF KIRALY

    124 126 134 138 144 150 156 162

    OUR SPOT DEVILOCK HANON SHOP DALEK JAKE CHRIS SILVA MIKE GENOVESE LINDA GREGORIOU

    168 174 180 186 192

    FUDGE FACTORY COMICS MARK BODE MEPHISTO JONES GWG INDEX

    A BIG THANKYOU TO _ PETER BURTON, MR PABLO FERRO, ALLEN FERRO AND JOY MORE, LEE QUINONES AND FAMILY AS WELL AS AMBER FOSSE, JOSE AND REY PARLA, DMOTE AND NICK UPSTAIRSCOLLECTIVE, KOSTAS SEREMITIS, JEST AT ALIFE, EMMET KEANNE AT ANSWER, JAKE, SHIKIKO IKEGAMI, MASAMICHI KATAYAMA AND KOZO KATAYAMA AT WONDERWALL , ANDY HOWELL, MIKE MILLS, ERIC NORMINGTON AND LANA K AT THE DIRECTORS BUREAU, PHILLIPE AND COMPUTERLOVE CREW, JUSTIN FOX, LINDA GREGORIOU, ANGELA BOATWRIGHT - KILLER OF GIANTS, MICHAEL C PLACE AND NIKI AS WELL AS BROCKMAN AND BETTY DESIGNBYBUILD.COM, HOST AND THE GLUESOCIETY, ANNICA AT DIRTYBANDITS.COM, MICHAEL GENOVESE AND CHRIS SILVA, MARK AND MOLLY BODE, JOHN AT STEREO, DION AND CLAIRE AT OURSPOT, LOUISE AND NICKY AT VILLAGE PRESS , BRIAN AT HANON, RUSS AND MG AT SSUR, HIDEKI INABA, AKIHIRO IKEGOSHI AT GWG KEEP ROCKIN, THE KING AT SKULL DEZAIN, NATH