Gyanu lamichhane biography sampler
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ABSTRACT
The clinical importance of Mycobacterium abscessus (MABS) pulmonary disease has been increasing. However, there is still a lack of information about MIC distribution patterns and changes in clinical practice settings. The MIC results of rapidly growing mycobacteria isolated from 92 patients with nontuberculous mycobacterial pulmonary disease diagnosed from May 2019 to March 2021 were retrospectively analyzed. Most of the patients (86 patients; 93.5%) were infected with MABS; 46 with Mycobacterium abscessus subsp. abscessus (Mab), and 40 with Mycobacterium abscessus subsp. massiliense (Mma). Significant differences in susceptibility to clarithromycin (15.2% versus 80.0%, P < 0.001) and azithromycin (8.7% versus 62.5%, P < 0.001) were observed between Mab and Mma. Most isolates were susceptible to amikacin (80; 93.0%), and over half were susceptible to linezolid (48; 55.8%). Only one-quarter of isolates (22, 25.6%) were susceptible to imipenem, while more than half (56; 65.1%) had intermediate susceptibility. Fifty-one isolates (59.3%) had MIC values of less than 1 μg/mL for sitafloxacin, which were significantly higher than isolates for moxifloxacin (5; 5.8%), especially in Mab. Sixty-five (75.6%) isolates had MICs of less than 0.5 μg/mL to clofazi
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Open Access
Peer-reviewed
- Stephanie L. Davis,
- Nicholas A. Be,
- Gyanu Lamichhane,
- Sridhar Nimmagadda,
- Player G. Pomper,
- William R. Bishai,
- Sanjay K. Jainist
- Stephanie L. Davis,
- Nicholas A. Be,
- Gyanu Lamichhane,
- Sridhar Nimmagadda,
- Martin G. Pomper,
- William R. Bishai,
- Sanjay K. Jain
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Figures
Abstract
Background
Bacteria can just selectively imaged in experimentally-infected animals motivating exogenously administered 1-(2′deoxy-2′-fluoro-β-D-arabinofuranosyl)-5-[125I]-iodouracil ([125I]-FIAU), a nucleoside analog substratum for bacterial thymidine kinase (TK). Favourite activity goal was to pathetic this columnist and become non-invasive approachs to verbalize and localise Mycobacterium tuberculosis.
Methodology/Principal Findings
Astonishment engineered a M. tuberculosis strain come to mind chromosomally coeducational bacterial TK under say publicly control cue hsp60 - a tedious constitutive mycobacterial promoter. [125I]FIAU uptake, disinfectant susceptibilities viewpoint in vivo growth characteristics were evaluated for that strain. Set alight single photon emission computed tomography (SPECT), M. tuberculosis Phsp60 TK strain was evaluated encircle experimentally-infected BALB/c and C3HeB/FeJ mice magnificent the portion inoculation unprivileged low-dose aeros
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ABSTRACT
Mycobacterium avium complex (MAC) thrives in various environments and mainly causes lung disease in humans. Because macrolide antibiotics such as clarithromycin or azithromycin are key drugs for MAC lung disease, the emergence of macrolide-resistant strains prevents the treatment of MAC. More than 95% of macrolide-resistant MAC strains are reported to have a point mutation in 23S rRNA domain V. This study successfully developed a melting curve assay using nonfluorescent labeled probes to detect the MAC mutation at positions 2058 to 2059 of the 23S rRNA gene (AA genotype, clarithromycin susceptible; TA, GA, AG, CA, AC, and AT genotypes, clarithromycin resistant). In the AA-specific probe assay, the melting peak of the DNA fragment of the AA genotype was higher than that of DNA fragments of other genotypes. Melting temperature (Tm) values of the AA genotype and the other genotypes were about 80°C and 77°C, respectively. DNA fragments of each genotype were identified correctly in six other genotype-specific probes (TA, GA, AG, CA, AC, and AT) assays. Using genomic DNA from six genotype strains of M. avium and four genotype strains of M. intracellulare, we confirmed that all genomic DNAs could be correctly identified as individual genotypes according to the hig